Peptide & GLP-1 Dosing Guides
Plain-English explainers for the concepts behind every dose — reconstitution, bacteriostatic water, reading an insulin syringe, GLP-1 titration and microdosing, half-life, cost, and stacking. Every guide links straight to the tool that does the math.
How to Reconstitute a Peptide
To reconstitute a peptide, slowly add bacteriostatic water down the side of the vial, let it dissolve without shaking, then divide the vial strength by the water volume to get a mg/mL concentration. Divide your dose by that concentration to find the volume, and multiply by 100 for units on a U-100 syringe.
Reconstitution & mixing
2How to turn a lyophilized vial into an accurate, drawable dose.
How to Reconstitute a Peptide
To reconstitute a peptide, slowly add bacteriostatic water down the side of the vial, let it dissolve without shaking, then divide the vial strength by the water volume to get a mg/mL concentration. Divide your dose by that concentration to find the volume, and multiply by 100 for units on a U-100 syringe.
Read guideBacteriostatic Water Explained
Bacteriostatic water is sterile water with about 0.9% benzyl alcohol added to inhibit bacterial growth, making it suitable for multi-dose vials. It is the standard diluent for reconstituting lyophilized peptides. The volume you add sets the concentration and how many units you draw, but never changes the milligram dose itself.
Read guideGLP-1 therapy
2Titration, microdosing, and dosing math for semaglutide, tirzepatide, and retatrutide.
GLP-1 Titration Basics
GLP-1 titration means starting at a low weekly dose and stepping up in fixed increments every few weeks. Community and anecdotal reports commonly describe semaglutide moving from about 0.25 mg toward 2.4 mg and tirzepatide from about 2.5 mg toward 15 mg. These are figures people report, not recommendations. Gradual escalation is described as giving the gut time to adapt.
Read guideGLP-1 Microdosing Explained
GLP-1 microdosing means using semaglutide, tirzepatide, or retatrutide at doses well below standard therapeutic targets, aiming for a gentler effect with fewer side effects. Protocols use either a slow low-dose ladder or an extended hold at a single small dose. Reconstituting with more water makes these tiny draws easier to measure.
Read guidePeptides & protocols
1Planning multi-compound protocols and research peptide dosing.
Dosing fundamentals
3The core concepts behind every dose: syringes, half-life, and cost.
Reading an Insulin Syringe: Units vs mL
On a standard U-100 insulin syringe, 100 units equal 1 mL, so each unit is 0.01 mL. To convert a dose volume to units, multiply the millilitres by 100. Insulin syringes come in 0.3 mL (30 units), 0.5 mL (50 units), and 1 mL (100 units) sizes; smaller barrels give finer, more accurate marks for small draws.
Read guideHalf-Life and Steady State for Dosing
Half-life is the time for the amount of a compound in your body to fall by half. A compound reaches about 97% of steady state after roughly five half-lives, regardless of how often you dose. Longer half-lives relative to the dosing interval mean more accumulation and a slower approach to a stable level.
Read guideCost per Dose Explained
Cost per dose is the vial price divided by the number of doses it yields, and the number of doses is the vial strength divided by your per-injection dose. A 10 mg vial dosed at 2 mg yields five doses, so a cost per dose is the vial price divided by five. Multiply by weekly frequency to project weekly and monthly spend.
Read guideFor educational and research purposes only. Not medical advice.